鎵嬫彁寮忚秴鑱叉尝绱�(x矛)鑳炵矇纰庢(j墨),DH-250鏄竴绋埄鐢ㄨ秴鑱叉尝鍦ㄦ恫楂斾腑鐢�(ch菐n)鐢熺┖鍖栨晥鎳�(y墨ng)鐨勫鍔熻兘銆佸鐢ㄩ€斿剙鍣�;瀹冭兘鐢ㄤ簬澶氱ó鍕�(d貌ng)妞嶇墿銆佺梾姣�銆佺窗(x矛)鑳�銆佺窗(x矛)鑿岀殑鐮寸锛屽悓鏅�(sh铆)鍙敤渚�(l谩i)涔冲寲銆佸垎鏁�銆佸嫽鍖�銆佹彁鍙�銆佹秷娉°€佹竻娲�銆佺磵绫虫潗鏂欑殑鍒跺倷銆佸垎鏁ｅ強鍔犻€熷寲瀛�(xu茅)鍙嶆噳(y墨ng)绛�銆�

鎵嬫彁寮忚秴鑱叉尝绱�(x矛)鑳炵矇纰庢(j墨)鏄柊娆剧敘(ch菐n)鍝侊紝鍔熻兘鍏�锛屾€ц兘鍙潬銆傚剙鍣ㄩ噰鐢↙ED椤ず锛屾湁鑷嫊(d貌ng)鍜屾墜鍕�(d貌ng)锛堣叧韪忥級鍏╃ó宸ヤ綔妯″紡锛岃秴鑱叉檪(sh铆)闁�銆佸姛鐜囦换鎰忚ō(sh猫)瀹�锛屾ǎ鍝佹韩搴︽娓�(c猫)椤ず锛岄牷鐜囪嚜鍕�(d貌ng)璺熻工銆�

鈼嗙敤浜庡嫊(d貌ng)妞嶇墿绱�(x矛)鑳�銆佺窗(x矛)鑿�銆佽娊瀛㈡垨绲勭箶鐨勭矇纰庯紝寰炵窗(x矛)鑳炰腑鎻愬彇铔嬬櫧璩�(zh矛)浠ュ強閫�(j矛n)琛岀梾姣�銆佺柅鑻楃殑绉戝(xu茅)鍩归(y菐ng)瀵�(sh铆)椹�(y脿n)銆�

鈼嗗姞閫熷寲瀛�(xu茅)銆佺墿鐞嗗(xu茅)绛夌殑鍙嶆噳(y墨ng)閫熷害鍜屽姞閫熸恫楂旇劔姘ｃ€�

鈼嗗師娌圭█閲�銆佹补姘翠钩鍖�銆佸姞閫熻劔鏅讹紝鐜荤拑鍧囨伎绛夐€�(j矛n)琛岀殑绉戠爺鍒嗘瀽銆�

鈼嗗垎鏁ｇ█鍦燂紝鍚勯鐒�(w煤)姗�(j墨)绀︾墿璩�(zh矛)锛屼互鍙婇厤鍒惰繎绱嶇背鐧惧垎涔嬩竴鐨勪钩鑶犻珨鍧囧寲娣峰悎鐗╃瓑銆�

鈼嗘ā鍏峰井瀛�锛岀洸瀛旂殑蹇€�?g貌u)?qi谩ng)鍔涢珮绮炬礂娑层€�

瀵�(sh铆)椹�(y脿n)?z膩i)搴�?/p>

1銆佷簡瑙ｈ秴鑱茬窗(x矛)鑳炵牬纰庣殑鍩烘湰鍘熺悊

2銆佹帉鎻¤秴鑱茬窗(x矛)鑳炵牬纰庣殑鍩烘湰鎶€琛�(sh霉)

瀵�(sh铆)椹�(y脿n)鍘熺悊

寮�(qi谩ng)鑱叉尝浣滅敤婧舵恫鏅�(sh铆)锛屾埃娉＄敘(ch菐n)鐢�銆侀暦(zh菐ng)澶у拰鐮寸鐨勭┖鍖栫従(xi脿n)璞℃湁闂�(gu膩n)锛岀┖鍖栫従(xi脿n)璞″紩璧风殑娌栨搳娉㈠拰鍓垁鍔涗娇绱�(x矛)鑳炶瑙ｃ€�

鏉愭枡鍜岃│鍔�

绱�(x矛)鑿�10ml銆佺噿鏉�銆佸埁鍐�銆�75锛呴厭绮炬鐞�銆�

绱�(x矛)鑳炵牬纰庣殑鏂规硶:

涓€銆佹(j墨)姊扮牬纰庢硶锛氭槸鎸囧埄鐢ㄦ悧纰庢(j墨)銆佺爺纾ㄥ櫒鎴栧嫽婕垮櫒 绛夊皣绱�(x矛)鑳炵牬纰庨枊(k膩i)渚�(l谩i) 銆�

1. 楂橀€熺祫绻旀悧纰庯細灏囨潗鏂欓厤鎴愮█绯婄媭娑�锛屾斁缃簬绛掑収(n猫i)绱�1/3楂旂⿳锛岃搵绶婄瓛钃�锛屽皣瑾�(di脿o)閫熷櫒鍏堟挜鑷虫渶鎱㈣檿锛岄枊(k膩i)鍕�(d貌ng)闁�(k膩i)闂�(gu膩n)鍚�锛岄€愭鍔犻€熻嚦鎵€闇€閫熷害銆傛娉曢仼鐢ㄤ簬鍕�(d貌ng)鐗╁収(n猫i)鑷熺祫绻�銆佹鐗╄倝璩�(zh矛)绋瓙绛�銆�

2. 鐜荤拑鍕绘伎鍣ㄥ嫽婕匡細鍏堝皣鍓鐨勭祫绻旂疆浜庣涓�锛屽啀濂楀叆鐮旀】渚�(l谩i)鍥炵爺纾�锛屼笂涓嬬Щ鍕�(d貌ng)锛屽嵆鍙皣绱�(x矛)鑳炵爺纰�锛屾娉曠窗(x矛)鑳炵牬纰庣▼搴︽瘮楂橀€熺祫绻旀悧纰庢(j墨)鐐洪珮锛岄仼鐢ㄤ簬閲忓皯鍜屽嫊(d貌ng)鐗╄嚐鍣ㄧ祫绻�銆�

浜屻€佺墿鐞嗙牬纰庢硶锛氭寚鍒╃敤婧害宸�銆佸鍔涘樊鎴栬秴鑱叉尝绛夊皣绱�(x矛)鑳炵牬纰庨枊(k膩i)渚�(l谩i)銆�

1锛氱敤涓€瀹氬姛鐜囩殑瓒呰伈娉㈣檿鐞嗙窗(x矛)鑳炴嚫娑�锛屼娇绱�(x矛)鑳炴€ュ妵闇囪暕鐮磋(鍊熷姪瓒呰伈鐨勯渿鍕�(d貌ng)鍔涚牬纰庣窗(x矛)鑳炲鍜岀窗(x矛)鑳炲櫒锛�銆�

姗�(j墨)鍒讹細鍙兘鑸囧挤(qi谩ng)鑱叉尝浣滅敤婧舵恫鏅�(sh铆)锛屾埃娉＄敘(ch菐n)鐢熴€侀暦(zh菐ng)澶у拰鐮寸鐨勭┖鍖栫従(xi脿n)璞℃湁闂�(gu膩n)锛岀┖鍖栫従(xi脿n)璞″紩璧风殑娌栨搳娉㈠拰鍓垁鍔涗娇绱�(x矛)鑳炶瑙�銆�

瓒呰伈娉㈢牬纰庣殑鏁堢巼鍙栨焙浜庤伈闋汇€佽伈鑳�銆佽檿鐞嗘檪(sh铆)闁�銆佺窗(x矛)鑳炴績搴﹀拰绱�(x矛)鑳為鍨嬬瓑銆傦紙浣跨敤鏅�(sh铆)娉ㄦ剰闄嶆韩锛岄槻姝㈤亷(gu貌)鐔憋級銆�

2. 楂樺鐮寸锛氱窗(x矛)鑳炴嚫娴恫寰為珮澹撳鐨勭挵(hu谩n)鐙€闅欏櫞灏勫埌闈滄鐨勬挒鎿婄挵(hu谩n)涓�锛岃杩敼璁婃柟鍚戠稉(j墨ng)鍑哄彛绠℃祦鍑�銆傛閬�(gu貌)绋嬩腑绱�(x矛)鑳炵稉(j墨ng)姝蜂簡楂橀€熼€犳垚鐨勫壀鍒囩殑纰版挒鍙婇珮澹撳埌甯稿鐨勮畩鍖�锛屽緸鑰岀牬纰庨噵鏀惧収(n猫i)鍚墿銆�

閫欐槸涓€绋韩鍜岀殑銆佸竟搴曠牬纰庣窗(x矛)鑳炵殑杓冪悊鎯崇殑鏂规硶銆�

3. 鍙嶅京(f霉)鍑嶈瀺娉曪細灏囩窗(x矛)鑳炲湪-20搴︿互涓嬪啺鍑�锛屽婧瀺瑙�锛屽弽寰�(f霉)骞炬锛岀敱浜庣窗(x矛)鑳炲収(n猫i)鍐扮矑褰㈡垚鍜屽墿浣欑窗(x矛)鑳炴恫鐨勯菇婵冨害澧為珮寮曡捣婧惰劰锛屼娇绱�(x矛)鑳炵祼(ji茅)妲�(g貌u)鐮寸銆�

涓夈€佸寲瀛�(xu茅)鐮寸娉曪細鎸囧埄鐢ㄧ敳閱�銆佷笝閰瓑鏈夋(j墨)婧跺姂鎴栬〃闈㈡椿鎬у姂浣滅敤浜庣窗(x矛)鑳炶啘锛屼娇绱�(x矛)鑳炶啘鐨勭祼(ji茅)妲�(g貌u)閬埌鐮村鎴栭€忔€х櫦(f膩)鐢熸敼璁� 銆�

鏈変簺鍕�(d貌ng)鐗╃窗(x矛)鑳�锛屼緥濡傝叓鐦ょ窗(x矛)鑳炲彲閲囩敤鍗佷簩鐑峰熀纾洪吀閳夛紙SDS锛夈€佸幓姘ц喗閰搁垑绛夌窗(x矛)鑳炶啘鐮村銆傛績搴︿竴鑸偤1mg/ml銆�

鍥�銆侀叾瀛�(xu茅)鐮寸娉� 锛氶伕鐢ㄥ悎閬╃殑閰讹紝浣跨窗(x矛)鑳炲閬埌鐮村锛岄€�(j矛n)鑰屽湪浣庢徊婧舵恫涓皣鍘熺敓璩�(zh矛)楂旂牬纰庨枊(k膩i)渚�(l谩i)銆�

绱�(x矛)鑿岀窗(x矛)鑳炲杓冨帤锛屽彲閲囩敤婧惰弻閰惰檿鐞嗘晥鏋滄洿濂姐€�

瑁傝В娑叉(bi膩o)婧�(zh菙n)閰嶆柟: 锛�50mM Tris-HCl(pH8.5~9.0), 2mM EDTA, 100mM NaCl, 0.5% Triton X-100, 1mg/ml婧惰弻閰躲€�(婧惰弻閰跺湪閫欏€�(g猫)pH鑼冨湇鍏�(n猫i)姣旇純濂界櫦(f膩)鎻綔鐢�) 銆�

缍滃悎鏁樿堪锛氱劇(w煤)璜栫敤鍝竴绋柟娉曠牬纰庣祫绻旂窗(x矛)鑳�锛岄兘鏈�(hu矛)浣跨窗(x矛)鑳炲収(n猫i)铔嬬櫧璩�(zh矛)鎴栨牳閰告按瑙ｉ叾閲嬫斁鍒版憾娑蹭腑锛屼娇澶у垎瀛愮敓鐗╅檷瑙�锛屽皫(d菐o)鑷村ぉ鐒剁墿璩�(zh矛)閲忕殑娓涘皯锛屽姞鍏ヤ簩鐣颁笝鍩烘盁纾烽吀锛圖FP锛夊彲浠ユ姂鍒舵垨娓涙參鑷憾浣滅敤锛涘姞鍏ョ涔欓吀鍙互鎶戝埗閭ｄ簺娲绘€т腑蹇冮渶瑕佹湁鐤忓熀鐨勮泲鐧芥按瑙ｉ叾鐨勬椿鎬�锛屽姞鍏ヨ嫰鐢茬：閰版盁鍖栫墿锛圥MSF锛変篃鑳芥竻闄よ泲鐧芥按瑙ｉ叾娲诲姏锛屼絾涓嶆槸鍏ㄩ儴锛岃€屼笖鎳�(y墨ng)瑭插湪鐮寸鐨勫悓鏅�(sh铆)澶氬姞骞炬锛涘彟澶�锛岄倓鍙€氶亷(gu貌)閬告搰pH銆佹韩搴︽垨闆㈠瓙寮�(qi谩ng)搴︾瓑锛屼娇閫欎簺姊濅欢閮借閬╁悎浜庣洰鐨勭墿璩�(zh矛)鐨勬彁鍙�銆�

浣跨敤鍓嶆敞鎰忎簨闋�(xi脿ng)锛�

1锛庢牴鎿�(j霉)鎵€铏曠悊鐨勬ǎ鏈珨绌嶉伕鎿囬仼鐣�(d膩ng)鎺㈤牠锛屼娇鐢ㄥ墠鍚庡潎闋堢敤閰掔簿妫夌悆鎿︽礂鎺㈤牠锛屾彌鎺㈤牠闋堝墰灏堢敤鎵虫墜鏇存彌锛�

2锛嶢MPLITUDE鏃嬮垥鎵撻枊(k膩i)鏅�(sh铆)锛屾帰闋笉寰楀湪绌烘埃涓毚闇�锛岀壒娈婃儏娉佷笅(娓�(c猫)瑭︽帰闋�)涓嶆噳(y墨ng)瓒呴亷(gu貌)10绉�锛�

3锛庝娇鐢ㄥ墠婧�(zh菙n)鍌欏ソ鍐扮洅锛屾ǎ鍝佽檿鐞嗘檪(sh铆)蹇呴爤缃簬鍐版荡涓�锛屾ǎ鍝佹績搴︿笉鍙亷(gu貌)澶с€�